immune response of foot and mouth disease virus type a87/irn inactivated vaccine by gamma irradiation on guinea pig in iran
نویسندگان
چکیده
foot and mouth disease (fmd) is the most contagious disease of cloven-hoofed animals, causingsignificant economical losses in livestock animals. in this study fmd virus type a87/irn was cultured andmultiplied on bhk21 cells. the fmd virus was titrated by the tissue culture infection dose50 method(tcid50 /ml), the virus titration was 107.5.. the fmd virus samples were irradiated and inactivated by gammaray from a 60co source at -20 °c. a safety test was done by the ibrs2 monolayer cell culture method, alsoantigenicity of irradiated and un-irradiated virus samples was studied by complement fixation test. thedose/survival curve for irradiated fmdv was drawn. according to the curve and d10 value factor, theoptimum dose range for the inactivation of fmdv type a87/irn and unaltered antigenicity was obtained(40-44 kgy). the inactivated virus samples by irradiation and ethyleneimine (ei) were formulatedrespectively as a vaccine with al (oh) 3 gel and other substances. the vaccines were inoculated to guineapigs and the results of the sero-neutralization test for both the normal vaccine and radio-vaccine showedprotective titre after 8 months. the potency test of the inactivated vaccines was done, protective dose50 value (pd50 value) of the vaccines were calculated to be 7.06 and 5.6 for the inactivated vaccine by ei and gamma irradiation respectively.
منابع مشابه
Immune Response of Foot and Mouth Disease Virus Type A87/irn Inactivated Vaccine by Gamma Irradiation on Guinea Pig in Iran
Foot and Mouth Disease (FMD) is the most contagious disease of cloven-hoofed animals, causing significant economical losses in livestock animals. In this study FMD Virus type A87/IRN was cultured and multiplied on BHK21 cells. The FMD virus was titrated by the Tissue Culture Infection Dose50 method (TCID50 /ml), the virus titration was 10. The FMD virus samples were irradiated and inactivated b...
متن کاملOrganic Circulation and persistence of Foot – and – Mouth disease virus type O in guinea pig
Since, the most susceptible laboratory animal to Foot–and–Mouth disease virus (FMDV) is guinea pig, and then one milliliter of FMDV type (O) concentrations of 106-106.5 TCID50was inoculated intradermally (ID) to 10 plantar surface of guinea pig (right side). Guinea pig adapted virus has been prepared after generalization phase, and then one milliliter of virus was inoculated intradermally to 30...
متن کاملEvaluation of cytokine mRNA expression in vaccinated guinea pigs with foot-and-mouth disease type O inactivated vaccine
Foot-and-mouth disease (FMD) is a severely contagious viral disease that mainly affects cloven-hoofedlivestock and wildlife. This study quantifies the cytokines mRNA expression of vaccinated guinea pigs withFMD type O inactivated vaccine. Blood samples were collected from eight guinea pigs at 7 and 28 days after thefirst vaccination. Extracted mRNAs were reverse-transcribed into cDNA and analyz...
متن کاملorganic circulation and persistence of foot – and – mouth disease virus type o in guinea pig
since, the most susceptible laboratory animal to foot–and–mouth disease virus (fmdv) is guinea pig, and then one milliliter of fmdv type (o) concentrations of 106-106.5 tcid50was inoculated intradermally (id) to 10 plantar surface of guinea pig (right side). guinea pig adapted virus has been prepared after generalization phase, and then one milliliter of virus was inoculated intradermally to 30...
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عنوان ژورنال:
iranian journal of science and technology (sciences)ISSN 1028-6276
دوره 31
شماره 1 2007
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